G-250 CBB staining buffer (500 mL)

Chemical Fina Conc Volume/Weight
Coomassie Brilliant Blue G-250 0.5 % [^conc of G-250] 2.5 g
Methanol 50% (v/v) 250 mL
Acetic acid 10% (v/v) 50 mL
ddH2O to final volume 200 mL

Actually, it is not the best recipe for G-250. This one works better for R-250. However, the chemical room dose not have R-250 so I have to prepare G-250 buffer.

[^conc of G-250]: Actually, the concentration generally differ from 0.052% to 0.1%. I used 0.5% here just for do a quick staining. I can only perform staining for 30 min or 1 hour and distaining overnight. It is important that this method is not good for weak bands.

30% CBB distaining buffer (500 mL)

Chemical Fina Conc Volume
Methanol 30% (v/v) 150 mL
Acetic acid 10% (v/v) 50 mL
ddH2O to final volume 200 mL