Regular Buffers in biomedical experiments

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1 M Tris-HCl (pH 7.9 @ 4°C), 500 mL

Reagents

• Tris: C4H11NO3, 121.136 g/mol
• HCl: for adjustment of pH

Procedure

• Weigh 60.568 g Tris. Dissolve in 450 mL water@4°C.
• Let it cool down to 4 °C.
• Adjust the pH to 7.9 @4°C with HCl.
• Add water until total volume is 500 mL.

0.5 M EDTA (pH 8.0), 500 mL

Reagents

• EDTA: C10H16N2O8, 292.244 g/mol

  EDTA·2H₂O: C10H18N2Na2O10, 372.24 g/mol

• NaOH: for adjustment of pH

Procedure

• Weigh 93.06 g EDTA·2H₂O. Dissolve in 400 mL water.
• Adjust the pH to 8.0 with NaOH.
• Add water until total volume is 500 mL.

2.5 M NaCl, 1000 mL

Reagents

• NaCl: 58.443 g/mol

Procedure

• Weigh 146.11 g EDTA·2H₂O. Dissolve in 900 mL water.

• Add water until total volume is 1000 mL.